Vol. 7, Issue 1, Part A (2025)

The present work is designed so as to evaluate its cyto-toxic effect on onion root tip cells. Onion root tips were placed in container containing cypermethrin in a solution for 24 hrs root tips absorbed along with the distilled water which has residual pesticide. These tips were cultured with varied concentrations of streptomycin for three to five days in root tip cells like c- metaphase. In instances of growth in onion root tip cell mitotic inhibition leaf extract of marigold act as g0 biological activity at higher treatment duration. Allium cepa is used as an in vitro plant for evaluating the effects of chemical agents. Onion chromosomes have also been prepared for culturing purposes by employing the centred stain technique using the aceto -arsenic squashing procedures root tips are cut from each bulb and soaking is done for 1,3days in Aceto arsenic stain and then cytologically examined. The study aimed to unveil if there is any correlation between the division rate of onion root tip cells during the treatment and the apportionment of aceto orcin over the relationship amongst the cells of the roots. The study aimed to find out chromosomal aberration & its impact on the mitotic index of onion root. The tip of onion root cells was observed going through mitosis while actively undergoing mitotic division using the usual methods.